Spec-seq: determining protein-DNA-binding specificity by sequencing
نویسندگان
چکیده
منابع مشابه
DNA specificity enhanced by sequential binding of protein monomers.
Transcriptional activation often requires the rapid assembly of complexes between dimeric transcription factors and specific DNA sites. Here we show that members of the basic region leucine zipper and basic region helix-loop-helix zipper transcription factor families follow an assembly pathway in which two protein monomers bind DNA sequentially and form their dimerization interface while bound ...
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The specificities of transcription factors are most commonly represented with probabilistic models. These models provide a probability for each base occurring at each position within the binding site and the positions are assumed to contribute independently. The model is simple and intuitive and is the basis for many motif discovery algorithms. However, the model also has inherent limitations t...
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ژورنال
عنوان ژورنال: Briefings in Functional Genomics
سال: 2014
ISSN: 2041-2649,2041-2657
DOI: 10.1093/bfgp/elu043